Marker Screening of the Early Diagnosis of Prostate Cancer in ...

Abstract

Objective:To Detect the content of prostate cancer and non-prostate cancer in patients with prostatic fluid survivin,?-methylacyl-coA racemase(AMACR), thymosin?15,nuclear matrix protein(NMP),analysis and comparison of various indicators of early diagnosis specificity and sensitivity,then screening prostate cancer marker of can increase the rate of early diagnosis,comprehensive analysis various indicators relations with prostate cancer staging and prognosis.Materials:64 example prostate cancer specimen comes from the Second Affiliated Hospital of Kunming Medical College urology patients(age above 50 years old, average age 58 years old ),Gleason graduation:?-?Level 27 examples,?-?Level 18 examples,?-?10 examples,?Level above 9 examples,24 example control group specimen from urology surgical in-patient and outpatient.Methods:Use clean centrifuge tube and the method of prostate massage to obtain prostatic fluid in patients with prostate cancer in 64 cases and non-prostate cancer patients 24 cases as a control group,the specimen are preserved puts inside-70?the refrigerator,use enzyme-linked immune response(ELISA) testing specimens At the same time.specimens were divided into two groups in accordance with whether bone metastases,each protein in bone metastasis group and no bone metastases using SPSS 13.0 software for statistical analysis of rank sum test;thymosin proteins?nuclear matrix protein?AMACR protein?survival protein data were divided into four groups:respectively is?-?grade group,?-?grade group,?-?grade group,?group level and above in accordance with the results of the pathology report,Enters the row rank and the examination statistical analysis these four groups with the SPSS 13.0 softwares.Results:1:the sensitivity of?-methylacyl-coA racemase in 64 example prostate cancer patient?s prostatic fluid is 96.9%(62/64),moreover is control group?s nearly 20 times,it has significance(P?0.01),only two example?-methylacyl-coA racemase density have no distinguished with the control group;64 example prostate cancer patient?s prostatic fluid thymosin?15 average density is 5 times more than non-prostate cancer?s,it?s sensitivity is 87.5%;64 example prostate cancer live albumen protein close normal 5 times,it?s sensitivity is 84.4%;The nuclear substrates protein average density is close control group?s 4 times,it?s sensitivity is 92%.2:Survivin protein have no correlation with prostate cancer staging and prognosis( P=0.83);?-methylacyl coenzyme A racemase have no correlation with prostate cancer staging and prognosis(P=0.192);nuclear matrix proteins have no correlation with prostate cancer staging and prognosis(P=0.449);Thymosin?15 has relevance with grading of prostate cancer(P?0.01).3:The thymosin?15 target have relevance with the prostate cancer?s shift(P?0.01 );the survivin protein target have no obvious relevance with prostate cancer?s shift( P=0.081);?-methylacyl coenzyme A racemase target have no obvious relevance with prostate cancer?s shift(P=0.217);the nuclear matrix proteins target have no obvious relevance with prostate cancer?s shift(P=0.226);the thymosin?15 target have relevance by stages with the prostate cancer(P?0.01).Conclusion:1:the sensitivity of?-formyl-coenzyme A racemase(AMACR) is 96.9%(62/64);the sensitivity of Thymosin?15 is 87.5%;the sensitivity of Survivin protein is 84.4%;the sensitivity of nuclear matrix protein is 92%.2:Thymosin?15 have relevance with transfer of prostate cancer.3:Thymosin?15 have relevance with grading of prostate cancer

Source: http://www.res-medical.com/oncology/66759

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